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Cells were collected via dissociation by TrypLE Express and pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes, followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes) and stored at -80C until further analysis

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GPCR signaling regulation: the role of GRKs and arrestins

To maximize the benefits of glutathione IV therapy , its recommended to continue hydrating, eat nutrient-rich foods, and follow any post-care instructions provided by your healthcare provider