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In this study, knockout of Munc18 abolished vesicular release and was lethal

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This is due to less stomach acid and intrinsic factor, making absorption harder

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In A549/ADR (DOX-resistant human lung adenocarcinoma cell) tumor-bearing nude mice, administration of DD-H/P NCs completely inhibited tumor growth, prolonged survival with an 80% survival rate, and significantly increased DOX accumulation in tumor sites compared with the free drug group, with no obvious toxicity in major organs [115]