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Injection Protocol and Frequency Standard protocol: One subcutaneous injection per day, 5 to 7 days per week
Cells were labeled with 2 M fluorescent nitric oxide indicator DFA-FM DA and fluorescence was detected by fluorescent microscope with a standard fluorescein bandpass filter (FITC, 500555 nm)
Allergic reactions, such as a rash, itching, swelling, or redness, happen due to high vitamin dosage or as a response to the needle coating
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Techniques such as meditation, deep breathing, or yoga can help manage stress, which often aggravates body aches and tiredness
Aside from hyperactivity, SHR rats are readily reported to exhibit low level of spontaneous alternation behavior in the Y-maze test [41, 42]
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