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The two copper ions in the conserved active site, CuA and CuB, are coordinated by six histidine residues 5,6,7

Nakai, K., & Tsuruta, D

doi: 10.3109/10715769409147509

One of the most common physiological changes seen is the depletion of the bodys most powerful antioxidant, reduced glutathione

The final predicted expression of both CBS OPT and L2HGDH OPT were MYPYDVPDYALQISCBS OPT (or L2HGDH OPT )DYKDDDDKKSRGPGRATGAAANSAVDGTAGPGSTGSRVDGSGEGRGSLLTCGDVEENPGP (with the features: HA tag, amino acid linker 1, CBS OPT (or L2HGDH OPT ), FLAG, amino acid linker 2, T2A self-cleavage site, in the order of N to C terminal) (#230088 and #230089, Addgene)