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Spas typically have these

To enable direct comparison across biofluids while accounting for matrix-dependent differences in signal intensity, plasma samples were injected at a lower plasma-equivalent volume to compensate for their higher metabolite concentrations 54,55

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High-sensitivity MRM measurements were then used for targeted analysis of modified peptides, using reference standards of all possible cysteine modified peptides

This protocol only provides a guideline, and should be modified according to your specific needs) [2]