All cells were cultured in 96-well plates (2.5 10 4 cells/ml) and different concentrations of Erastin and QCT were at 0 h, 24 h, 36 h, and 48 h after plating, CCK-8 reagent (10 l) was added to the wells, followed by a 2-h incubation at 37 C
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Furthermore, being the study cross-sectional, possible prospective cohort studies should be developed in the future to actually establish the supposed causal relationships
Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M
Wilson, D
Together, these data demonstrate that ablation of GSH synthesis in mice results not only in reduced expression of lipogenic enzymes but also lower levels of their corresponding lipid species