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Gradient elution was performed: initially 15% B for 0.50 min, linear increase to 90% B for 1.0 min, constant at 90% B for 0.50 min, back to 15% B in 0.10 min and constant at 15% B for 0.40 min

3Inject the diluent into your research vial, directing the stream against the glass wall to avoid foaming

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