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D57 (CTR) and D254 (G), were predicted protonated, however, visual inspection did not support this prediction because of their solvent-exposed location and, therefore, were modelled in the anionic side chain form

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Numerous studies have demonstrated that MYB TFs, upon activation by environmental stimuli, can bind to cis-acting elements in the promoters of downstream stress-responsive genes or interact with other proteins to fine-tune their expression, ultimately enhancing plant tolerance to abiotic stress