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Abbreviations ASL: Airway surface liquid ALI: Airliquid interface COPD: Chronic obstructive pulmonary disease WHO: World health Organization PM: Particulate Matter AED: Aerodynamic equivalent diameter ROS: Reactive oxygen species RNS: Reactive nitrogen species NAC: N-acetylcysteine hNEC: Human nasal epithelial cell poly I:C: Polyinosinic:polycytidylic acid HDM: House dust mite BaP: Benzo[a]pyrene CYP27B1: 1-Hydroxylase RSV: Respiratory syncytial virus hBEC: Human bronchial epithelial cell LPS: Lipopolysaccharide BALF: Bronchoalveolar lavage fluid TH17: T helper 17 DEP: Diesel exhaust particle EMT: Epithelial-mesenchymal transition CSE: Cigarette smoke extract GSH/GSSG: Glutathione (reduced/oxidized) CF: Cystic fibrosis IPF: Idiopathic pulmonary fibrosis References Yang W

Coefficient of Variance Intra Variation% 10% Inter Variation% 12% Recovery 95-102% Linearity Diluent Ratio Range % 1:2 93-105 1:4 88-106 1:8 86-108 Specificity/Cross-reactivity No significant cross-reactivity or interference between GSSG and analogues was observed